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    Thermo Fisher poweru sybr green master mix
    Poweru Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/poweru+sybr+green+master+mix/agarose+chip+kit/pm37355987-183-19-25
    Average 90 stars, based on 1 article reviews
    poweru sybr green master mix - by Bioz Stars, 2026-10
    90/100 stars

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    Methylation:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.

    CCK-8 Assay:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.

    SYBR Green Assay:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.

    Retroviral:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.

    Real-time Polymerase Chain Reaction:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.

    Homologous Recombination:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.

    Electrophoretic Mobility Shift Assay:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.

    Recombinant:

    Article Title: A cancer-associated METTL14 mutation induces aberrant m6A modification, affecting tumor growth.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-METTL3 antibody Proteintech Cat# 15073-1-AP, RRID:AB_2142033 Rabbit polyclonal anti-METTL14 antibody Sigma-Aldrich Cat# HPA038002, RRID:AB_10672401 Mouse monoclonal anti-MET antibody Cell Signaling Technology Cat# 3127, RRID:AB_331361 Rabbit monoclonal anti-phospho-MET Cell Signaling Technology Cat# 3077, RRID:AB_2143884 Rabbit monoclonal anti-MYC antibody Cell Signaling Technology Cat# 5605, RRID:AB_1903938 Rabbit monoclonal anti-GAPDH antibody Cell Signaling Technology Cat# 2118, RRID:AB_561053 Rabbit polyclonal anti-m6A antibody Synaptic Systems Cat# 202 003, RRID:AB_2279214 Goat Anti-Mouse IgG (HL)-HRP Conjugate Bio-Rad Cat# 172–1011, RRID:AB_11125936 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Cat# 172–1019, RRID:AB_11125143 Biological samples Human Genomic DNA (Demale) Promega Cat# G1521 Chemicals, peptides, and recombinant proteins Foretinib Selleck Cat# GSK1363089 Actinomycin D Cayman Chemical Cat# 11421 RIPA Lysis and Extraction Buffer Thermo Scientific Cat# 89900 Trans-Blot Turbo Transfer Pack Bio-rad Cat# 1704156 SuperScript IV VILO Master Mix Thermo Fisher Scientific Cat# 11755050 FastStart Universal Probe Master Roche Cat# 4913957001 Hoechst Invitrogen Cat# H21486 RNA Fragmentation Reagents Thermo Fisher Scientific Cat# AM8740 QIAzol Lysis Reagent QIAGEN Cat# 79306 RNaseOU Recombinant Ribonuclease Inhibitor Invitrogen Cat# 51535 SuperScrip III Reverse Transcriptase Invitrogen Cat# 18080093 NEBNext Magnesium RNA Fragmentation Module New England Biolabs Cat# E6150 Puromycin Dihydrochloride Thermo Fisher Scientific Cat# A1113803 Lipofectamine 2000 DNA Transfection Reagent Thermo Fisher Scientific Cat# 11668-019 Lenti-X concentrator Clontech Cat# 631231 RNaseA Promega Cat# A797C Bst 2.0 DNA Polymerase New England Biolabs Cat# M0537 SplintR ligase New England Biolabs Cat# M0375 Recombinant YTHDF2 protein ActiveMotif Cat# 31573 T4 Polynucleotide Kinase New England Biolabs Cat# M0201S Adenosine 5’-triphosphate, [g-32P]- PerkinElmer Cat# NEG002A nuclease P1 Fujifilm Wako Pure Chemical Industries Cat# 145-08221 bacterial alkaline phosphatase Takara Cat# 2120A Critical commercial assays Poly(A)Puris MAG Kit Thermo Fisher Scientific Cat# AM1922 FastGeneTM RNA Premium Kit NIPPON genetics Cat# FG-81250 QIAamp DNA mini kit Qiagen Cat# 51306 GenElute mRNA Miniprep Kits Sigma Cat# MRN70-1KT (Continued on next page) 14 Cell Reports 42, 112688, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER EpiQuik m6A RNA Methylation Quantification Kit EpiGentek Cat# P-9005-48 Cell Counting Kit-8 Dojindo Cat# CK04 PowerU SYBR Green Master Mix Applied Biosystems Cat# A25742 Deposited data International Cancer Genome Consortium (ICGC) N/A https://dcc.icgc.org/ Catalog of Somatic Mutations in Cancer (COSMIC) https://doi.org/10.1093/nar/gky1015 https://cancer.sanger.ac.uk/cosmic Raw MeRIP-Seq data This paper GEO: GSE190112 Experimental models: Cell lines Human: HEC108 Dr. Etsuko Kajimoto N/A Platinum-GP Retroviral Packaging Cell Line Cell Biolabs Cat# RV-103, RRID:CVCL_B490 Experimental models: Organisms/strains BALB/cAJcl-nu/nu CLEA N/A Oligonucleotides qPCR primer for c-MYC Thermo Fisher Scientific Cat# Hs00153408_m1 qPCR primer for c-MET Thermo Fisher Scientific Cat# Hs01565584_m1 qPCR primer for GAPDH Thermo Fisher Scientific Cat# Hs02758991_g1 Single-stranded DNA oligonucleotide for homologous recombination Hokkaido System Science N/A RNA oligonucleotide for electrophoretic mobility shift assay (EMSA) Hokkaido System Science N/A Recombinant DNA pSpCas9 BB-2A-GFP GenScript Cat# PX458 I.M.A.G.E. .. Full Length cDNA clone (METTL14) DnaForm Clone ID: 3010654 pMSCVpuro vector Clontech Cat# 631461 pCMV-VSV-G Riken Cat# RDB04392 Software and algorithms Tide https://doi.org/10.1093/nar/gku936 https://tide.nki.nl/ GSEA N/A https://www.gsea-msigdb.org/gsea/index. jsp MEME-ChIP https://doi.org/10.1093/bioinformatics/ btr189 https://meme-suite.org/meme/tools/ meme-chip CentriMo https://doi.org/10.1093/nar/gks433 https://meme-suite.org/meme/doc/ centrimo.html Trim-galore https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ Tophat https://doi.org/10.1093/bioinformatics/ btp120 https://ccb.jhu.edu/software/tophat/index. shtml Samtools https://doi.org/10.1093/bioinformatics/ btp352 http://www.htslib.org/doc/samtools.html MetPeak R https://doi.org/10.1093/bioinformatics/ btw281 https://github.com/compgenomics/ MeTPeak exomePeak R https://doi.org/10.1016/j.ymeth.2014.06.



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